Oscar Gonzales, head of the High Technology Crime Division, told The Associated Press that slain Peruvian business student Stephany Flores did not use van der Sloot's laptop to research the Holloway case.
Van der Sloot is a longtime suspect in the disappearance of the Alabama teen, who was on spring break in Aruba in 2005 when she vanished. He is accused in the 2010 slaying of Flores. Investigators believe she was killed May 30 -- exactly five years after Holloway disappeared.
The Dutchman has been charged with first-degree murder and robbery in the Flores case. If convicted, he could face 15 to 35 years in prison.
Van der Sloot has also been indicted by U.S. authorities, accused of a plot to extort $250,000 from Holloway's family in exchange for information on her death and the location of her body.
Earlier this month, van der Sloot's attorney, Maximo Altez, said he plans to argue temporary insanity in the case, claiming van der Sloot became enraged when he found out that Flores had used his laptop to look up information on Holloway. That explanation, however, may not hold up if Gonzales is correct in his assertion that Florez had conducted no such searches.
Peruvian authorities reportedly provided the FBI with a copy of the laptop hard drive last week. The FBI is believed to be searching for information on the disappearance of Holloway and the alleged extortion of money from the Holloway family.
"We deny the colonel's claim," Altez told the AP. "We will be presenting our own expert analysis regarding the laptop." He did not specify what it might show.
Radio Netherlands Worldwide has reported that Peruvian computer experts discovered that someone using the laptop after Flores' murder had searched for information on countries that do not have extradition treaties with Latin American nations.
A spokesman for the FBI's Birmingham, Ala., field office told AOL News that he could neither "confirm nor deny" that it is investigating the contents of the laptop.